Purification and Characterization of a Thermostable Lipase from Geobacillus thermodenitrificans IBRL-nra
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Purification and Characterization of a Thermostable Lipase from Geobacillus thermodenitrificans IBRL-nra
Thermostable lipase from Geobacillus thermodenitrificans IBRL-nra was purified and characterized. The production of thermostable lipase from Geobacillus thermodenitrificans IBRL-nra was carried out in a shake-flask system at 65°C in cultivation medium containing; glucose 1.0% (w/v); yeast extract 1.25% (w/v); NaCl 0.45% (w/v) olive oil 0.1% (v/v) with agitation of 200 rpm for 24 hours. The extr...
متن کاملOrganic-Solvent and Surfactant Tolerant Thermostable Lipase, Isolated from a Thermophilic Bacterium, Geobacillus thermodenitrificans IBRL-nra
Lipases (triacylglycerol acylhydrolase, EC 3.1.1.3), catalyze the hydrolysis of long-chain triglycerides with the formation of diacylglyceride, monoglyceride, glycerol and fatty acids. Lipases are actively used in various industries which include food and diary, pharmaceuticals, organic synthesis and detergent and cosmetics. Thermostable lipases are commercially significant for their potential ...
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Isolation of novel thermophilic bacilli has received considerable attention among the scientific community in whole world because of their biotechnological importance as they possess unique enzymes with thermal activity and stability. Of the thermophilic enzymes, we were interested in α-glucosidases which catalyze hydrolysis of terminal non-reducing α-D-glucosidic linkages of oligoand polysacch...
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The lantibiotic nisin has been used as an effective food preservative to combat food-borne pathogens for over 40 y. Despite this successful use, nisin's stability at pH 7 is limited. Herein, we describe a nisin analog encoded on the genome of the thermophilic bacterium Geobacillus thermodenitrificans NG80-2. This analog termed geobacillin I was obtained by heterologous expression in Escherichia...
متن کاملPurification and Characterization of a Thermostable Laminarinase from Penicillium rolfsii c3-2(1) IBRL
A laminarinase (endo-β-1,3-glucanase) was purified to homogeneity from Penicillium rolfsii c3-2(1) IBRL, which was originally produced in liquid culture containing 1% xylan from birchwood, via anion-exchange chromatography, gel filtration on Sephacryl S-100, and hydrophobic interaction chromatography. A single protein band with a molecular weight of 75 kDa was detected by sodium dodecyl sulfate...
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ژورنال
عنوان ژورنال: Enzyme Research
سال: 2012
ISSN: 2090-0406,2090-0414
DOI: 10.1155/2012/987523